Control vector: RPL10_PROM
类别:分子生物学试剂 货号:S708908 10ug
人气:4 品牌:Switchgear Genomics
价格:¥5,400 规格:10 ug
关键字: 产地:美国
种属: - 检测范围: -
表达系统(蛋白): - 抗体来源种属: -

SwitchGear offers a number of control constructs to optimize experimental conditions and to normalize for non-specific effects associated with a treatment or change in condition. Note that SwitchGear’s unique RenSP luciferase technology eliminates the need for co-transfection of a normalizing control in most cases.

Empty promoter vector: The empty promoter vector contains the RenSP luciferase gene without a promoter. This construct serves as a one measure of background signal in the experiment. This construct may also be used to create additional experimental constructs.

Housekeeping gene promoter vectors: Housekeeping control constructs contain promoters for common housekeeping genes driving the RenSP luciferase reporter. These constructs serve as positive transfection controls and may also serve as controls for comparing signals between conditions if they are known to be unresponsive to the test condition.

Random control vectors: Random control constructs contain 1 kb non-conserved, non-genic, and non-repetitive fragments from the human genome cloned upstream of the luciferase reporter. These vectors produce slightly different signals than the empty vectors and are considered optimal negative or background controls.

promoter chart Figure 1. Expected data and analysis of housekeeping and random promoter vectors: Random promoter constructs (R1, R2, R3, R4) serve as negative/background controls and establish baseline luciferase activity. The housekeeping promoter controls serve as positive controls and give relative luciferase activity.
Y-axis values shown in log scale. Control vector activity may vary depending on cell line, treatment condition, and protocol changes.
Empty Promoter Vector
联系我们时,请说明是在生物港上看到的,谢谢。
公司 所在地 价格 购买 详情
百为生物技术有限公司 上海-上海市 5,400 立即购买 查看
评论
您未登录,不能评论!
促销商家
    浏览记录