MICROFIL Silicone Rubber Injection Compound (四色)
类别:其他试剂 货号:Kit D
人气:12 品牌:Flow Tech
价格:¥11,280 规格:kit
关键字: 产地:美国
种属: - 检测范围: -
表达系统(蛋白): - 抗体来源种属: -

 

 

MICROFILâ compounds will fill and opacify microvascular and other spaces of non-surviving animals and post-mortem tissue under physiological injection pressure. The continuous, closed vascular system lends itself to flow through injection or perfusion techniques.  Following injection, MICROFIL compounds cure to form a three-dimensional cast of the vasculature.

MICROFIL® MV-series compounds are available in five radiopaque colors, as well as clear.  MV-series compounds require either an alcohol-methyl salicylate or glycerin clearing sequence, whereby the refractive index of the clearing solution is the same as the refractive index of the tissue.  This allows for microscopic examination of a selected vascular bed.

MICROFIL® CP-101 compound is intended for use in cast-corrosion techniques, and is designed for filling large blood vessels (greater than 100 microns).  Although the CP-101 compound will fill capillaries, these vessels fragment when the supporting tissue is removed through exposure to a potassium hydroxide solution.  When cured, MICROFIL® CP-101 is a milky white in color.  Casts made using CP-101 will maintain their dimensional accuracy indefinitely.

Advantages offered with MICROFIL® compounds over previously available rubber injection materials include:

Complete filling with minimal shrinkage, to enhance vessel continuity and to produce in cleared preparations a vivid, optically cleared specimen that allows a precise study of the microcirculation.

Color diversity, to provide delineation within the circulatory tree for microscopic examination and photographic illustration,

Areas of investigation

In physiology, MICROFIL® visualization provides a means for establishing the precise vascular architecture of specific organs, allowing comparison between normal and abnormal structure.

In surgery, visualization of the microcirculation and microanatomy is leading to improved surgical techniques in the repair of nerves, tendons, and blood vessels.

In gastrointestinal research, MICROFIL® compounds characterize and describe changes in vascular patterns associated with several pathological conditions.

Injected specimens, when preserved in methyl salicylate or glycerin, also serve as a definitive teaching adjunct.

MV-series mixing procedure

To achieve a viscosity level suitable for injection of the microcirculation, it is necessary to blend the MV compound with an equal quantity (by weight) of MV-Diluent. Volume mixing requires 5ml of diluent for every 4ml of compound.  The mixture of compound and diluent is catalyzed with 5% (by weight or volume) of MV Curing Agent.  Viscosity ranges from 20 to 30 centipoise.  Working time is 20 minutes and begins with the addition of curing agent.

Table 1

Physical properties of MICROFIL® compound

 

MV-112

MV-117

MV-120

MV-122

MV-130

MV-132

MV-Diluent

CP-101

         

Color:

White

Orange

Blue

Yellow

Red

Clear

Clear

Milky White

Specific gravity:

1.04

1.02

1.00

1.04

1.02

1.00

0.92

0.98

Viscosity, centipoise:

35

25

25

25

30

20

5

25

Gel time, minutes:

90

90

90

90

90

90

 -

45

Useful shelf life in Months:

4

4

4

4

4

4

Indefinite

6

Notes;
1. Viscosity measured with a Brookfield Model LVF Viscometer, with a No. 2 spindle at 30RPM.
2. Gel time measured on a blend (by weight) of one part MV compound and one part MV-Diluent followed by addition of 5% MV Curing Agent.  Gel time is time required for mixture to cease flowing.
3.  Shelf life is in excess of four months.  For materials held beyond four months, it is possible to run the following static test to determine acceptability:
        a. Mix 5 grams of MV compound with 5 grams of MV-Diluent in a vial.
        b. Add 10 drops (medicine dropper) of MV Curing Agent.
        c. Cap, shake, and refrigerate overnight.
        b. The mixture should gel in the vial after this procedure to assure a cure in subsequent
            animal injections.

Catalyzed mixtures will form an elastomeric gel after 90 minutes at room temperature.  Curing takes place with non-exothermic cross-linking and minimal volume change.

It has been possible to refrigerate specimens immediately after injection and still obtain complete cure after overnight aging.  The procedure decreases odor level for subsequent sectioning.


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