Agencourt AMPure XP, 60 mL
类别:分子生物学试剂 货号:A63881
人气:2,452 品牌:Beckman
价格:¥3,588 规格:60ml
关键字:PCR纯化系统 产地:美国
种属: - 检测范围: -
表达系统(蛋白): - 抗体来源种属: -

The Agencourt AMPure XP system is a highly efficient, easily automated PCR purification system that delivers superior quality DNA with no salt carryover. Requiring no centrifugation or filtration, Agencourt AMPure XP can be easily used in manual and automated 96- or 384-well formats.
Agencourt AMPure XP - thumb

Application: PCR purification

Downstream Application Techniques: PCR, genotyping (SNP detection) fragment analysis, sequencing (Sanger and Next Generation), cloning, primer walking

Agencourt AMPure XP Features
  • High recovery of amplicons, greater than 100 bp
  • Efficient removal of unincorporated dNTPs, primers, primer dimers, salts and other contaminants
  • Stable PCR products post-cleanup: No PCR degradation after storage at 4° C for seven days
  • Efficient recovery of double stranded and single stranded DNA templates
  • Consistent recovery throughout the kit’s 12 month shelf life
  • Faster manual and automated processing as compared to traditional post-PCR cleanup methods
Process Overview
Agencourt AMPure XP process overview

1. PCR reaction 2. Binding of PCR amplicons to magnetic beads 3. Separation of PCR amplicons bound to magnetic beads from contaminants 4. Washing of PCR amplicons with Ethanol 5. Elution of PCR amplicons from the magnetic particles 6. Transfer away from the beads into a new plate

High Recovery

Agencourt AMPure XP purification system recovers amplicons greater than 100 bp with consistency and high reproducibility. The recovery efficiency of the Agencourt AMPure XP kit for small and large amplicon sizes is superior to the traditional filtration PCR cleanup methods (Figure 1).

Agencourt AMPure XP process figure

Figure 1. The recovery of the PCR products post-PCR cleanup was measured by spectrophotometry; 200 bp, 800 bp and 10 Kb PCR products where purified with the Agencourt AMPure XP kit, QIAquick* kit and QuickStep* filtration system.

Walk-away Automated Solution

Agencourt AMPure XP post-PCR cleanup has been automated on three Beckman Coulter liquid handlers, the Biomek 3000, NXP and FXP for processing scalability. The LIMS compatible automation functionality is aided by a user-friendly interface equipped with a quick startup menu, a deck setup walk-through menu, a method recovery menu, and a procedure menu.

公司 所在地 价格 购买 详情
济南翔宇生物 山东省-济南市 3,588 立即购买 查看
百为生物技术有限公司 上海-上海市 9,980 立即购买 查看
上海益同生物科技有限公司 上海-上海市 9,980 立即购买 查看
上海起发实验试剂有限公司 上海-上海市 15,600 立即购买 查看