(PsbD / Anti-D2 subunit of Photosystem II, N-terminal antibody)
Rabbit polyclonal antibody
The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex.
KLH-conjugated synthetic peptide derived from N-terminal of PsbD in Arabidopsis thaliana ATCG00270.
Serum Peptide affinity form antibody available upon request at firstname.lastname@example.org.
Reconstitution with 150 µl of sterile water. "Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70℃ as supplied. 6 months, -20 to -70℃ under sterile conditions after reconstitution. 1 month, 2 to 8℃ under sterile conditions after reconstitution.
The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.
Western Blot (1:1000-1:2000) Note: Optimal dilutions/concentrations should be determined by the end user.
39.5 / 33 kDa
Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Indica Group, Glycine max, Solanum tuberosum, Cucumis sativus, Sorghum bicolor, Nicotiana tabacum, Setaria viridis, Populus trichocarpa, Vitis vinifera, Medicago truncatula, and 80-99% homologues with the sequence in Spinacia oleracea, Triticum aestivum, Hordeum vulgare subsp. Vulgare, Physcomitrella patens. For more species homologues information, please contact tech support at email@example.com.
1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. Electrophoresis: 15% SDS-Urea-PAGE Transfer: blotting to NC (nitrocellulose) membrane for 1h. Blocking: 5% skim milk at RT or 4℃ for 1h. Primary antibody: 1:2000 dilution overnight at 4℃. Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000) Detection:using chemiluminescence substrate and image were captured with CCD camera.