PsbD植物抗体,PHY0090S  (PsbD / Anti-D2 subunit of Photosystem II, N-terminal antibody)
类别:抗体 货号:PHY0090S
人气:23 品牌:PhytoAB
价格:¥2,890 规格:150ug
关键字:PsbD抗体,植物抗体,PhytoAB 产地:美国
种属: - 检测范围: -
表达系统(蛋白): - 抗体来源种属: -

Product Information

  Description:

Rabbit polyclonal antibody

  Background:

The D1/D2 (PsbA/PsbD) reaction center heterodimer binds P680, the primary electron donor of PSII as well as several subsequent electron acceptors. D2 is needed for assembly of a stable PSII complex.

  Synonyms:

PsbD

  Immunogen:

KLH-conjugated synthetic peptide derived from N-terminal of PsbD in Arabidopsis thaliana ATCG00270.

  Form:

Lyophilized

  Quantity:

150 µg

  Purification:

Serum
Peptide affinity form antibody available upon request at info@phytoab.com.

  Reconstitution:  

Reconstitution with 150 µl of sterile water. 
"Note: please spin tube briefly prior to opening it to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tube".

  Stability &     
  Storage:

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 
12 months from date of receipt, -20 to -70℃ as supplied.
6 months, -20 to -70℃ under sterile conditions after reconstitution. 
1 month, 2 to 8℃ under sterile conditions after reconstitution.

  Shipping:

The product is shipped at 4℃. Upon receipt, store it immediately at the temperature recommended above.

 

Application Information

  Recommended Dilution: 

Western Blot (1:1000-1:2000) 
Note: Optimal dilutions/concentrations should be determined by the end user.

  Expected/apparent MW: 

39.5 / 33 kDa

  Confirmed Reactivity: 

Arabidopsis thaliana

  Predicted Reactivity: 

Among 25 analyzed species, the sequence of the synthetic peptide used for immunization is 100% homologues with the sequence in Oryza sativa Indica Group, Glycine max, Solanum tuberosum, Cucumis sativus, Sorghum bicolor, Nicotiana tabacum, Setaria viridis, Populus trichocarpa, Vitis vinifera, Medicago truncatula, and 80-99% homologues with the sequence in Spinacia oleracea, Triticum aestivum, Hordeum vulgare subsp. Vulgare, Physcomitrella patens.
For more species homologues information, please contact tech support at tech@phytoab.com.

 

Application Example

1-3 is thylakoid membrane protein from Arabidopsis thaliana leaf containing 0.1 μg, 0.25 μg, and 0.5 μg of chlorophyll, respectively. 
Electrophoresis: 15% SDS-Urea-PAGE
Transfer: blotting to NC (nitrocellulose) membrane for 1h.
Blocking: 5% skim milk at RT or 4℃ for 1h.
Primary antibody: 1:2000 dilution overnight at 4℃.
Secondary antibody: 1:20000 dilution using Goat Anti-Rabbit IgG H&L (HRP) (Cat# PHY6000)
Detection: using chemiluminescence substrate and image were captured with CCD camera.

产品链接:http://www.phytoab.com/psbd-anti-d2-subunit-of-photosystem-ii-n-terminal-antibody?___SID=U

欲了解产品详情,欢迎致电北京启维益成科技有限公司400-810-0506

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