PI3K活性检测试剂盒  (PI 3-Kinase Assay Kit)
类别:其他试剂 货号:PR-943
人气:68 品牌:Jena Bioscience
价格:¥5,640 规格:96T
关键字:磷脂酰肌醇3激酶,Phosphoinositide 3-Kinase 产地:德国
种属: - 检测范围: -
表达系统(蛋白): - 抗体来源种属: -

说明书: 

Kit contents
2x Reaction Buffer (2 x 1.5 ml)
1mM ATP (400 l)
Lipid Substrate (8 tubes)
Control PI 3-Kinase (5 g)
Description
PI 3-Kinase Assay Kit provides a highly efficient and easy-to-handle tool for the sensitive detection and quantification of PI 3-Kinase activity or the screening for PI 3-Kinase inhibitors. The activity of PI3-Kinases is measured by the amount of radioactively labeled [32P]-ATP incorporated into lipid substrate. The substrate is thereafter separated using thin layer chromatography (TLC) and its radioactivity detected in a scintillation counter.
Background
Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes that play a pivotal role in important cellular regulatory mechanisms. PI3Ks are capable of phosphorylating the 3’-OH position of phosphoinositide lipids (PIs) generating lipid second messengers. Their function has been linked to the regulation of numerous biological processes including cell growth, differentiation, survival,
proliferation, migration. On the basis of structural similarities and substrate specificity, the PI3K family is divided into three classes termed I, II, and III. All human class I members are heterodimers consisting of a catalytic subunit (MW approx. 110 kDa) and a
non-catalytic subunit (MW 50, 55, 85, 87 or 101 kDa). They are known to phosphorylate phosphatidylinositol (PI), phosphatidylinositol-4-mono-phosphate (PIP) and phosphatidylinositol-4,5-bisphosphate (PIP2) in vitro but have a strong preference for PIP2 in vivo.

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